Endothelial cell culture from human umbilical cord vein
نویسندگان
چکیده
منابع مشابه
study of effects of cyclic tensile loading on morphology of human umbilical vein endothelial cell line in culture medium
introduction: endothelial cells are subjected to mechanical forces caused by pulsatile flow and pressure leading to hemodynamic shear stress and circumferential stress on endothelial layer. the study aimed at a quantitative evaluation of endothelial cell morphology by application of cyclic loading. endothelial cells respond to applied loads with adaptation and remodeling. such mechanisms are ma...
متن کاملEffect of Channel Width on Human Umbilical Vein Endothelial Cell (HUVEC) Culture in Microfluidic Channels
Capillary electrophoresis is a powerful separation technique that can achieve high efficiency separations of charged analytes. However, one limitation of capillary electrophoresis is the adsorption of cationic proteins and peptides onto the inner surface of the capillary wall, due to the negatively charged silanol groups on the bare fused silica capillaries. This can lead to poor separation eff...
متن کاملHypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis.
The conventional photosensitizers used in photodynamic therapy (PDT), such as haematoporphyrin (HP), have not yet reached satisfactory therapeutic effects on port-wine stains (PWSs), due largely to the long-term dark toxicity. Previously we have showed that hypericin exhibited potent photocytotoxic effects on Roman chicken cockscomb model of PWSs. However, the molecular mechanism of hypericin-m...
متن کاملEndothelial progenitor cell culture and differentiation in vitro: a methodological comparison using human umbilical cord blood.
OBJECTIVE Endothelial progenitor cells (EPC) can contribute to vascular repair and targeted tumour therapy. Little is known about generating EPC from human umbilical cord blood. We therefore compared methods for purification of EPC from human umbilical cord blood. METHODS Mononuclear cells were isolated from human umbilical cord blood by density gradient centrifugation and used either unselec...
متن کاملThrombin-mediated release of factor VIII antigen from human umbilical vein endothelial cells in culture.
We have examined the effects of purified human alpha-thrombin on factor VIII antigen (FVIII-Ag) release by human umbilical vein endothelial cells in culture. Alpha-thrombin induced a time and dose-dependent release of FVIII-Ag into supernatant medium. Alpha-thrombin-mediated FVIII-Ag release was not dependent on protein synthesis and was observed in both serum-free and serum-containing media. F...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Medical Journal of Indonesia
سال: 1994
ISSN: 2252-8083,0853-1773
DOI: 10.13181/mji.v3i2.944